Castilla y León is expanding the restriction perimeter for Avian Influenza in the area where outbreaks have occurred, with the objective of “encapsulating” the six AI outbreaks recorded in Valladolid by extending the restriction zone from 10 to 20 km
The Ministry of Agriculture, Fisheries and Food (MAPA) announced this Monday, 27 October 2025, the expansion of the restriction zones for Highly Pathogenic Avian Influenza (HPAI) H5N1) in Castilla y León to 20 kilometres in order to reinforce surveillance and biosecurity measures.

The measure, agreed between the Official Veterinary Services (SVO) of the Junta de Castilla y León, MAPA and the European Commission, establishes an Additional Restriction Zone (ZRA) that extends the security perimeter from 10 to 20 kilometres around the notified outbreaks, in accordance with Regulation 687/2020.
This Additional Restriction Zone (ZRA) affects the municipalities of Olmedo and Aguasal, where six outbreaks have occurred (from 19 September to 15 October) affecting a total of 1,970,000 laying hens, and the 10+10 km radius around the outbreak in the municipality of Ataquines (where on 20 October the most recent officially confirmed AI outbreak occurred) affecting 54,000 broilers. In total, AI has affected 2,024,251 poultry in the province of Valladolid, of which 97.3% are laying hens. Across the whole of Spain, as of 27 October 2025, the total number of farm birds affected now stands at 2,696,951.
Reinforcement of controls and biosecurity
In this new zone, stricter sanitary control and surveillance measures will be applied, including:
- Prior authorisation from the SVO for any movement of poultry within or to/from the ZRA.
- Increased active and passive surveillance on poultry holdings.
- Biosecurity assessments and enhanced controls through a specific disease-adapted questionnaire, following EFSA recommendations.
- Reinforced cleaning and disinfection controls for poultry transport vehicles operating in the zone.
- Restocking conditional upon an individualised risk analysis, taking into account poultry density, distances and biosecurity measures.

Call for vigilance across the sector
MAPA emphasises the importance of maintaining adequate biosecurity measures to prevent direct or indirect contact with wild birds, as well as the need to strengthen passive surveillance in both domestic poultry and wildlife. Any suspicion must be reported immediately to the Official Veterinary Services (SVO).
Tracking of Avian Influenza cases on poultry farms in Spain in 2025:
-. 1st outbreak of 2025 (18 July, Extremadura): Alert over an Avian Influenza outbreak on a 7,000-turkey farm in Extremadura
-. 2nd outbreak (28 July, Toledo): Second AI outbreak detected, this time in breeder hens in Toledo
-. 3rd outbreak (1 Sept., Huelva): The spectre of H5N1 returns to Andalusia: 8,500 turkeys culled due to avian influenza in Huelva.
-. 4th outbreak (8 Sept., Guadalajara): Spain records its fourth AI outbreak of 2025, this time on a breeder farm in Guadalajara
-. 5th outbreak (9 Sept., Huelva): H5N1 positive confirmed on the farm of 8,400 poultry in Valverde del Camino, Huelva, Andalusia
-. 6th outbreak (19 Sept., Valladolid): H5N1 AI detected on a 760,000-laying hen farm in Valladolid
-. 7th, 8th and 9th outbreaks (1 Oct., Valladolid and Madrid): 552,000 laying hens affected by three new AI outbreaks in Madrid and Valladolid
-. 10th outbreak (6 Oct., Valladolid): Tenth Avian Influenza outbreak detected in Spain. More than 800,000 laying hens to be culled.
-. 11th outbreak (13 Oct., Valladolid): Eleventh Avian Influenza outbreak detected in Spain on a farm of 66,000 laying hens.
-. 12th outbreak (15 Oct., Valladolid): 315,000 new laying hens affected in the latest outbreak
-. 13th outbreak (16 Oct., Toledo): 112,000 laying hens in Yeles, Toledo
-. 14th outbreak (20 Oct., Valladolid): 54,000 broilers in Valladolid
No new outbreaks have been recorded on poultry farms since 20 October (updated as of 31 Oct. 2025)

